Nanion portapatch planar patch clamping automate scientific. Instead of positioning a pipette on an adherent cell, cell suspension is pipetted on a chip containing a microstructured aperture. Microfluidic system for planar patchclamp electrode arrays xiaohui li yale university 2006 the patch clamp has been widely accepted as a standard technique for fundamental studies of ion channel proteins, and discovery of drugs that affect these proteins. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data throughput offered by available platforms is still far too low. The heart of the syncropatch 384i, the patch clamp module designed and produced by nanion technologies, is integrated into the stateoftheart liquid handling robot, the biomek i5, from beckman coulter. For patchliner experiments, a patchliner octo nanion technologies was used, including heka epc10 quadro amplifiers heka elektronik, lambrecht.
Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a highthroughput manner. Planar patch clamp definition of planar patch clamp by. With conventional patch clamp this is difficult because of the tiny dimensions of the pipette tip, the long tapered shaft and, thus, the diffusion limitation of the liquid exchange. Comparison of a typical pipettebased patch clamp rig on the top with a planar patch clamp rig on the bottom. A new technique for multiple reuse of planar patch clamp. These new technologies are designed to overcome many of the hurdles associated with traditional wholecell voltage clamp by dramatically reducing time and expertise required to perform. Planar arraybased automated patch clamp platforms include multiple commercially available instruments dunlop et al. Instead of recording currents using glass pipettes, planar arraybased platforms utilize a planar chip containing multiple micropores that. Not only a powerful research tool but also ideal for educational purposes and quick tests of cells and ion channels. One of these breakthroughs is the development of automated planar patchclamp technology. Planar patch clamp devices, such as the three described in this unit, are overtaking glass pipets in popularity because they increase throughput, are easier to use, provide for the acquisition of high. However, many of the protocols and notes given in this chapter can be applied to other automated patchclamp platforms. For patchliner experiments, a patchliner octo nanion.
Planar patch clamp approach to characterize ionic currents. Planar patch clamp synonyms, planar patch clamp pronunciation, planar patch clamp translation, english dictionary definition of planar patch clamp. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Due to their planar chip design not only is a higher throughput achieved, but new applications have also become possible. These planar patch clamp systems have properties of a high throughput, the automatic formation of a high seal resistance, the involvement of voltage and current clamps, and the capability of wholecell and single channel recordings, drug perfusion and data analysis. One of the advantages of planar patch clamp is the possibility of perfusing the intracellular side of the membrane during a patch clamp experiment in the wholecell configuration. Nanion technologies smart tools for ion channel research, drug. Npc16 patchliner and syncropatch 96 nanion technologies gmbh.
Nanion portapatch applications automate scientific. Here we describe features of nanions npc16 patchliner and processes and protocols suited for this particularly flexible and successful highthroughput automated platform, which is based on planar patchclamp technology. A relevant question is, however, should automated patch clamp technology be used in primary screening. Planar patch clamp synonyms, planar patch clamp pronunciation, planar patch clamp translation, english dictionary definition of planar. For the patch clamp recordings, planar borosilicate glass patch clamp chips were used 18 in a 384microtiterplate format. Nanions high quality instruments employ planar patch clamp chips which replace the traditional glass pipette used in the conventional patch clamp technique. It offers fast and easy access to high quality patch clamp data with only minimal. There is a book patchclamp analysis advanced techniques, chapter 14 planar patch clamping, which i think would help you. Planar patch clamp approach to characterize ionic currents from.
The syncropatch 384i is a high performance automated patch clamp apc system for recording from up to 384 wells simultaneously, with the possibility of upgrading to an unprecedented 768 wells. Instrumentation based on the planar patch clamp principle is made available by nanion technologies gmbh. A unique feature of nanion s automated patch clamp platforms is the possibility to exchange intracellular components. Lysosomal preparation and the planar patch clamp approach used for. The characterization of drug effects on human currents using automated patch clamp apc platforms employing single or multihole planar patch techniques 1,2,3,4,5 has. Fluorescence measurements using an upright microscope can therefore be combined with current recordings. Ampio pharmaceuticals announces agreement to acquire key delivery technology. Whole cell patch clamp recording performed on a planar glass chip. We have developed an automated patch clamp module for.
Cell suspensions for apc devices utilizing planar patch clamp chips should be homogeneous milligan et al. Using automated patch clamp electrophysiology platforms in. Nanion offers the portapatch microscope slide developed for the simultaneous visualization and patch clamp recording of the cell. Automated patch clamp meets highthroughput screening. Planar patch clamping, as the name implies, is a twodimensional or planar variation on traditional patch clamping. Does anyone use the automated patch clamp, the portapatch. As the first planar patch system with true gigaseal performance, patchxpress 7000a automated patch clamp system revolutionized ion channel drug discovery. Nowadays, the planar patch clamp systems are being developed toward. The patchliner is a fully automated planar patch clamp instrument recording from up to. Data output and compound information is compatible with most database formats. Sep 02, 2014 vendors of automated electrophysiology instruments make recommendations on cell lines suitable for their instruments, and cell line vendors provide validation data of their lines used on planar patch clamp systems. In 2009 nanion was awarded the german founders award grunderpreis, and the innovation prize step award. Nanions planar patch clamp devices the portapatch is a semiautomated device with a small footprint for the analysis of ion channels in cells and lipid bilayers.
Typically, cho and hek tumorigenic cell lines overexpressing single ion channels are. Typically, cho and hek tumorigenic cell lines overexpressing single ion channels are used since they can be harvested as highdensity, homogenous, singlecell. Automated electrophysiological and pharmacological. Npc1 chips single use, disposable microstructured glass chip containing an aperture of 1. Frontiers development of automated patch clamp technique to. We found no significant difference in patch clamp parameters such as cell capacitance and series resistance between automated and. However, the planar patch clamp platforms on the market today are far away from replacing the common work horses of primary ion channel screening.
The cells easily formed gigaohm seals, and patch clamp recordings were obtained from the wholecell configuration membrane holding potential 60 mv, applying 500 ms depolarizing voltage pulses. Systematic data production has been possible for a. Levis and rae, 1993 and is thus a very suitable substrate for planar patch clamp chips. Situated in the heart of munich, nanion is vibrant team of scientists and engineers with the goal of providing top quality instrumentation and customer service for a broad range of analytical applications. The impact of automated patch clamping apc on ion channel screening is now. Nanion has been the first on the market with the launch of the semiautomated planar. A mpc involves the use of a glass pipette that is manoeuvred to an adherent cell approach. Aug 07, 2015 384 or 768 cells recorded in parallel 20,000 data points per day 8595 % success rates routinely obtained reliable data from diverse ion channels targets seamless integration into. The module is incorporated into a laboratory pipetting robot and uses a 384channel pipettor head for application of cells and compounds. We miniaturized this patch clamp system even further by integrating the amplifier into the system. Planar patch clamp is a novel method developed for high throughput electrophysiology. The highthroughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. We have found that robotic multiwell planar patchclamp technology can generate high quality publishable data1012 and illustrative data shown in this article from native cells and cells in primary culture, as well as confer advantages and be enabling compared with conventional patchclamp.
Using automated patch clamp electrophysiology platforms in painrelated ion channel research. Only references contain multiwell planar patchclamp data. The herg safety service is performed at our parent company, evotec, and is a cellbased assay which employs the qpatch htx system sophion bioscience as or the syncropatch 384pe nanion technologies as automated patch clamp electrophysiology measurements. Nanions syncropatch 96 convinces big pharma automated patch. High gigaseal rate on hek, cho, ltk, rbl, ips and rbc. Automated patch clamp systems have recently been developed, in order to collect large amounts of data inexpensively in a shorter period of time. Robotic multiwell planar patchclamp for native and.
Our planar lipid bilayers are obtained from guvs prepared with the nanion vesicle prep pro setup. Planar patch clamp definition of planar patch clamp by the. Nanions instruments use planar patch clamp chips, which replace the traditional glass pipette used in the technique of patch clamp electrophysiology. One of these breakthroughs is the development of automated planar patchclamp. For many years now manual patchclamping has been regarded as one of the. A relevant question is, however, should automated patch clamp technology be. Nanions portapatch is not only the worlds smallest patchclamp setup with minimum foot print and low maintenance requirements, but also the cheapest way to access apc technology. Highthroughput automated patch clamp complemented by. For planar patch clamp, a wide range of different cell suspensions can be used bruggemann et al. It offers fast and easy access to high quality patch clamp data with only minimal training. Kcurrents were identified in the neural mouse stem cells as shown in the raw data traces below. Supporting gigaseal recordings from one cell at a time the portapatch mini offers fast and easy access to high quality patch clamp data with only minimal training. Unlike the plasma membrane, intracellular membranes are usually not stable enough to withstand mechanical manipulation by glass electrodes during seal formation and rupturing of the.
Since it was founded in 2002, nanion has grown to one of the most respected automated patch clamp providers worldwide. Frontiers development of automated patch clamp technique. Using automated patch clamp electrophysiology platforms in paina. Introduction he opening and closing of ion channels in cell membranes is the basis of many cell functions. The microelectrode pipette, whose aperture provides the suction that couples the instrument to the cell, is replaced by a planar electrode substrate into which a hole has been bored. We have developed an automated patch clamp module for highthroughput ion channel screening, recording from 384 cells simultaneously. The wholecell mode of the planar patchclamp technique portapatcho nanion, munich. The system uses nanions planar patch clamp chips and generates high quality data. Patchcontrol software nanion technologies portapatch nanion technologies software for data acquisition patchmaster, heka instruments inc. A planar patch clamp device with an accessible aperture is ideally suited for these kinds of combined experiments by which new insights on ion channel behavior can be gained. Following the successful market introduction of two automated patch clamp devices, the porta patch 2004 and the patchliner 2006, nanion now introduces a massively parallel patch clamp platform, the. The technique is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells, and can also be applied to the study of bacterial ion channels in.
Patch clamp recordings in the whole cell configuration, and single channel recordings in the cell attached configuration, can be performed with planar patch. Patch clamp recordings in the whole cell configuration, and single channel recordings in the cell attached configuration, can be performed with planar patch clamp chips. The portapatch mini is built on the success of the portapatch. The patchliner is an automated patch clamp robot for medium thoughput with ultimate flexibility for assay design.
Experiments are performed on a single cell at a time, whereby whole dose response curves can be obtained from a single cell. Indeed, synaptic transmission, or the relaying of signals from one neuron to another, is regulated by the opening and closing of these transmembrane proteins 1. The patch clamp technique is a laboratory technique in electrophysiology used to study ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane. Nanion technologies and data analysis datacontrol 384, nanion technologies. Automated electrophysiological and pharmacological evaluation. D population data for current amplitudes at 100 mv obtained from. A single cell is then positioned on the hole by suction and a tight connection gigaseal is formed. Crosssite and crossplatform variability of automated. One of the advantages of planar patch clamp is the possibility of perfusing the intracellular side. We thank nanion technologies for good technical support. Early identification of herg liability in drug discovery. Since its launch in the early 1980s, the patch clamp method has been extensively used to study ion channels in the plasma membrane, but its application to the study of intracellular ion channels has been limited. May 22, 2005 nanion s product is based on fertigs research, which bezanilla and other scientists praise highly.
Robotic multiwell planar patchclamp for native and primary. Munich, germany, november 10, 2012 nanions automated patch clamp platforms are known for their high cell compatibility, even for primary cells and stem cells. Systematic data production has been possible for a reasonably large number of cells on one day of. Portapatch planar patch clamping the portapatch is a onecellatatime system, which is surprisingly small for a complete patch clamp rig.
In 2009, nanion lauched its high throughput, highquality automated patch clamp system, the syncropatch 96. We employ planar microstructured borosilicate glass chips nanion technologies gmbh, munich, germany, used in a commercially available patchclamp workstation portapatch from nanion, which commonly is used for automated patchclamp recording purposes alone 16,17. Due to its dielectric properties quartz is the almost ideal material for patch clamp pipettes rae and levis, 1992. Renaissance of ion channel research and drug discovery by. Whole cell patch clamp recording performed on a planar. With its vast experimental freedom and gigaseal data quality, the patchliner is one of the most versatile patch clamp instruments on the market. Planar patch clamp chips with a chip resistance of 23. Molecular devices, axon instrument, sophion bioscience, cytocentrics and nanion technologies have already launched their planar patch clamp systems separately. Review development of planar patch clamp technology and its application in the analysis of cellular electrophysiology peihua chena, wei zhanga, jun zhoua, ping wanga, lidan xiaob, mo yangb abiosensor national special laboratory, key laboratory of biomedical engineering of education ministry, department of biomedical engineering, zhejiang university, hangzhou 310027, china.
Nanions syncropatch 384768pe is a high throughput planar patchclamp platform that achieves high and robust success rates. Because of its high data quality, robust recordings and superior level of. The patch clamp goes planar the scientist magazine. Traditional patch clamp has a very low throughput which has been proven to be a.
Nov 30, 2011 nanions high quality instruments employ planar patch clamp chips which replace the traditional glass pipette used in the conventional patch clamp technique. Such systems typically include a singleuse microfluidic device, either an injection molded or a polydimethylsiloxane pdms cast chip, to capture a cell or cells, and an integrated electrode. Using optimized conditions, we recorded both wt and corrected f508delcftr clcurrents with automated wholecell patch clamp and compared the data to results obtained with conventional manual wholecell patch clamp. We here report the development of an automatic device for whole cell patch clamping that can easily be. The device consists of a planar chip made from fused quartz. Nanions portapatch, claims to be the worlds smallest patchclamp workstation, and uses nanions planar patch clamp chips providing data on any ion channel target. Automated platforms have demonstrated the ability to generate highquality data with high throughput capabilities, at great ef fi ciency and reliability. Automated patch clamp meets highthroughput screening product news dec 01, 2016 a new research report published in the december 2016 issue of the journal of laboratory automation jala features a planar patch clamp system with modular design capable of recording up to 384 cells simultaneously.
Frontiers stateoftheart automated patch clamp devices. Nanions planar patch clamp chips offer the unique possibility to perfuse the. The paradigm shift from cellattached mode in oocytes to wholecell mammalian planar patch clamp was first published by niels fertig and colleagues at nanion. Dec 17, 2008 nanion s instruments use planar patch clamp chips which replace the traditional glass pipette used in the technique of patch clamping. Nanions product is based on fertigs research, which bezanilla and other scientists praise highly. During a recent demonstration of the portapatch in the department of. Development of planar patch clamp technology and its. Highthroughput automated patch clamp complemented by optical. Patchliner versatile and sophisticated the patchliner is a fully automated planar patch clamp instrument recording from up to 8 cells simultaneously.
It has been discussed whether cell lines based on cho cells or hek cells provide more relevant data wisniowska and polak, 2009. Microfluidic system for planar patchclamp electrode arrays. Contact is then made with the cell, and through application of negative pressure, an electrical seal forms, in the order of g. Development of planar patch clamp technology and its application in. Automated patch clamping finally achieves high throughput. For the patch clamp recordings, planar borosilicate glass patch clamp chips were used18 in a 384microtiterplate format.
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